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Neuroscience
Neurology Process
Growth & Development
Axonal Guidance Proteins
Anti-ROBO1 Antibody (A83460)
Overview
Specifications
Images
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$480
Product Datasheet
Goat polyclonal antibody to ROBO1 for ELISA, WB, IHC, IF and Flow Cytometry.
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Product Size:
100µg
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Shipping Information
Delivery
Freight Charges
$40 to United States
Lead Time
3-5 Business Days
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Specifications
Name
Anti-ROBO1 Antibody
Description
Goat polyclonal antibody to ROBO1.
Applications
ELISA
,
WB
,
IHC
,
IF
,
Flow Cytometry
Reactivity
Human
Immunogen
Synthetic peptide corresponding to Human ROBO1 (internal region).
Sequence
C-GDVDLSNKINEMK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100 µg at 0.5 mg/ml.
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 0.5% BSA.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Deleted in U twenty twenty, DUTT 1, DUTT1, FLJ21882, H Robo 1, H-Robo-1, hRobo 1, Robo 1, ROBO1_HUMAN, Roundabout 1, Roundabout axon guidance receptor homolog 1, Roundabout homolog 1, Roundabout homolog1 precurser, Roundabout1, SAX 3, SAX3
Isotype Controls
Goat IgG (A121671)
Suitable Secondaries
Donkey Anti-Goat IgG H&L Antibody (AP) (A300679)
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A300716)
Donkey Anti-Goat IgG H&L Antibody (FITC) (A300685)
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300730)
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (4)
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Figure 1: Western Blot - Anti-ROBO1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) (1µg/ml) staining of HUVEC lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Figure 2: Immunohistochemistry - Anti-ROBO1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Figure 3: Immunofluorescence - Anti-ROBO1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml), showing Golgi and membrane staining.
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Figure 4: Flow Cytometry - Anti-ROBO1 Antibody (A83460)
Anti-ROBO1 Antibody (A83460) - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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