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Anti-S100 beta Antibody [ARC50351] (A307742)

Western Blot - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.com Western Blot - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.com Immunohistochemistry - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.com Immunohistochemistry - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC50351] antibody to S100 beta for WB, IHC and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
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Email
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Name
Anti-S100 beta Antibody [ARC50351]
Description
Rabbit monoclonal [ARC50351] antibody to S100 beta.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-92 of human S100B (NP_006263.1).
Sequence
MSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDNDGDGECDFQEFMAFVAMVTTACHEFFEHE
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC50351
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
11 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
NEF, Protein S100 B, Protein S100-B, S 100 calcium binding protein beta chain, S 100 protein beta chain, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 calcium binding protein beta (neural), S100 calcium-binding protein B, S100 protein beta chain, S100B, S100beta, S100B_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-S100 beta Antibody [ARC50351] (A307742)
Western blot analysis of extracts of various cell lines, using Anti-S100 beta Antibody [ARC50351] (A307742) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-S100 beta Antibody [ARC50351] (A307742)
Western blot analysis of extracts of various cell lines, using Anti-S100 beta Antibody [ARC50351] (A307742) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-S100 beta Antibody [ARC50351] (A307742)
Immunohistochemistry analysis of paraffin-embedded human colonic peripheral nerve using Anti-S100 beta Antibody [ARC50351] (A307742) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-S100 beta Antibody [ARC50351] (A307742) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-S100 beta Antibody [ARC50351] (A307742)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-S100 beta Antibody [ARC50351] (A307742) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Publishing research using Anti-S100 beta Antibody [ARC50351] (A307742)? Please let us know so that we can list the citation on this page.

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