Anti-SAFB Antibody [ARC2983] (A309462)

Name: Anti-SAFB Antibody [ARC2983]
Brand: Antibodies.com
SKU: A309462

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This product is discontinued

Anti-SAFB Antibody [ARC2983] (A309462) has been discontinued and is no longer available.

View all Anti-SAFB Antibodies.

Specifications

Name

Anti-SAFB Antibody [ARC2983]

Description

Rabbit monoclonal [ARC2983] antibody to SAFB.

Applications

WB, IHC

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein of Human SAFB.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2983

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

150 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp779C1727, glutathione S transferase fusion protein, HAP, HET, HSP27 ERE TATA binding protein, HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, SAF B, SAF-B, SAF-B1, SAFB 1, SAFB1, SAFB1_HUMAN, Scaffold attachment factor B1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SAFB Antibody [ARC2983] (A309462)

Western blot analysis of extracts of various cell lines, using Anti-SAFB Antibody [ARC2983] (A309462) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-SAFB Antibody [ARC2983] (A309462)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-SAFB Antibody [ARC2983] (A309462) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SAFB Antibody [ARC2983] (A309462)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-SAFB Antibody [ARC2983] (A309462) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SAFB Antibody [ARC2983] (A309462)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-SAFB Antibody [ARC2983] (A309462) at a dilution of 1:50(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.