Anti-Sarcomeric Alpha Actinin Antibody [ARC1360] (A307576)

Western blot analysis of extracts of various cell lines, using Anti-Sarcomeric Alpha Actinin Antibody [ARC1360] (A307576) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of rat heart using Anti-Sarcomeric Alpha Actinin Antibody [ARC1360] (A307576) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of mouse heart using Anti-Sarcomeric Alpha Actinin Antibody [ARC1360] (A307576) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).