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Anti-SC35 Antibody (A11667)

Western Blot - Anti-SC35 Antibody (A11667) - Antibodies.com Western Blot - Anti-SC35 Antibody (A11667) - Antibodies.com Immunohistochemistry - Anti-SC35 Antibody (A11667) - Antibodies.com Immunohistochemistry - Anti-SC35 Antibody (A11667) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to SC35 for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-SC35 Antibody
Description
Rabbit polyclonal antibody to SC35.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SRSF2 (NP_003007.2).
Sequence
MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHH
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
28 kDa / 32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
35 kDa, arginine/serine-rich 2, PR264, Protein PR264, SC 35, SC-35, Serine/arginine-rich splicing factor 2, SFRS 2, SFRS2, SFRS2A, Splicing component, Splicing component 35 kDa, Splicing factor, Splicing factor arginine/serine rich 2, Splicing factor SC35, Splicing speckle, Splicing speckles, SR splicing factor 2, SRp30b, SRSF2, SRSF2_HUMAN
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-SC35 Antibody (A11667) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-SC35 Antibody (A11667)
Western blot analysis of extracts of mouse lung, using Anti-SC35 Antibody (A11667) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Western Blot - Anti-SC35 Antibody (A11667) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-SC35 Antibody (A11667)
Western blot analysis of extracts of various cell lines, using Anti-SC35 Antibody (A11667) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-SC35 Antibody (A11667) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-SC35 Antibody (A11667)
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-SC35 Antibody (A11667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-SC35 Antibody (A11667) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-SC35 Antibody (A11667)
Immunohistochemistry analysis of paraffin-embedded human lung using Anti-SC35 Antibody (A11667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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