Anti-Smad3 Antibody [ARC53858] (A306182)

Name: Anti-Smad3 Antibody [ARC53858]
Brand: Antibodies.com
SKU: A306182

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This product is discontinued

Anti-Smad3 Antibody [ARC53858] (A306182) has been discontinued and is no longer available.

View all Anti-Smad3 Antibodies.

Specifications

Name

Anti-Smad3 Antibody [ARC53858]

Description

Rabbit monoclonal [ARC53858] antibody to Smad3.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:20,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Smad3 (NP_005893.1).

Sequence

HHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53858

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZP586N0721, DKFZp686J10186, hMAD 3, hMAD-3, hSMAD3, HSPC193, HST17436, JV15 2, JV15-2, JV152, LDS1C, LDS3, MAD (mothers against decapentaplegic Drosophila) homolog 3, MAD homolog 3, Mad homolog JV15 2, Mad protein homolog, MAD, mothers against decapentaplegic homolog 3, Mad3, MADH 3, MADH3, MGC60396, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD, SMAD 3, SMAD family member 3, SMAD, mothers against DPP homolog 3, SMAD3_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Smad3 Antibody [ARC53858] (A306182)

Western blot analysis of various lysates, using Anti-Smad3 Antibody [ARC53858] (A306182) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-Smad3 Antibody [ARC53858] (A306182)

Western blot analysis of Rat testis, using Anti-Smad3 Antibody [ARC53858] (A306182) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Western Blot - Anti-Smad3 Antibody [ARC53858] (A306182)

Western blot analysis of extracts from wild type(WT) and Smad3 knockout (KO) HCT116 cells, using Anti-Smad3 Antibody [ARC53858] (A306182) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Immunohistochemistry - Anti-Smad3 Antibody [ARC53858] (A306182)

Immunohistochemistry analysis of paraffin-embedded human liver (negative control sample) using Anti-Smad3 Antibody [ARC53858] (A306182) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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