Anti-SMAD5 Antibody [ARC0448] (A308830)

Name: Anti-SMAD5 Antibody [ARC0448]
Brand: Antibodies.com
SKU: A308830
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0448] antibody to SMAD5 for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-SMAD5 Antibody [ARC0448]

Description

Rabbit monoclonal [ARC0448] antibody to SMAD5.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:500-1:1,000

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Smad5 (Q99717).

Sequence

STYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMDTSNNMIPQIMPSISSRDVQPVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFT

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0448

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-SMAD5 Antibody [ARC0448] (A308830)

Western blot analysis of extracts of K-562 cells, using Anti-SMAD5 Antibody [ARC0448] (A308830) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-SMAD5 Antibody [ARC0448] (A308830)

Western blot analysis of extracts of C2C12 cells, using Anti-SMAD5 Antibody [ARC0448] (A308830) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-SMAD5 Antibody [ARC0448] (A308830)

Immunoprecipitation analysis of 300µg extracts of K-562 cells using 3µg of Anti-SMAD5 Antibody [ARC0448] (A308830). This Western blot was performed on the immunoprecipitate using Anti-SMAD5 Antibody [ARC0448] (A308830) at a dilution of 1:1000.

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