Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476)

Name: Anti-SMAD5 (phospho Ser465) Antibody [ARC59204]
Brand: Antibodies.com
SKU: A309476
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC59204] antibody to SMAD5 (phospho Ser465) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-SMAD5 (phospho Ser465) Antibody [ARC59204]

Description

Rabbit monoclonal [ARC59204] antibody to SMAD5 (phospho Ser465).

Applications

Dilutions

WB: 1:1,000-1:5,000

Reactivity

Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S465 of human Smad5.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59204

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, JV5-1, MAD homolog 5, MAD, mothers against decapentaplegic homolog 5, MADH 5, MADH5, Mothers against decapentaplegic homolog 5, mothers against decapentaplegic, drosophila, homolog of, 5, Mothers against DPP homolog 5, MusMLP, SMA and MAD related protein 5, SMAD 5, SMAD family member 5, SMAD, mothers against DPP homolog 5, SMAD5_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476)

Western blot analysis of NIH/3T3, using Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476) at 1:2,000 dilution. NIH/3T3 cells were treated by BMP4(50ng/ml) at 37°C for 6 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476)

Western blot analysis of C6, using Anti-SMAD5 (phospho Ser465) Antibody [ARC59204] (A309476) at 1:2,000 dilution. C6 cells were treated by MG132(50 µM) and TGFbetaat 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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