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Anti-SNAI1 Antibody

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Total Citations (31)
Rabbit polyclonal antibody to SNAI1 from ABclonal (A5544).

   Please Note: This is exactly the same antibody that is marketed as:


 
   •  Abcam | ab180714
Name: Anti-SNAI1 Antibody
Description: Rabbit polyclonal antibody to SNAI1.
Applications: WB, IHC
Dilutions: WB: 1:500 - 1:2000, IHC: 1:50 - 1:100.
Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein of human SNAI1.
Host: Rabbit
Clonality: Polyclonal
Isotype: IgG
Conjugate: Unconjugated
Purification: Affinity purification.
Product Form: Liquid
Formulation: Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Function: Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
Tissue Specificity: Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
Sequence Similarities: Belongs to the snail C2H2-type zinc-finger protein family.
Post-Translational Modification: Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Cellular Location: Nucleus. Cytoplasm.

Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
UniProt: O95863
Gene ID: 6615
Synonyms: dJ710H13.1 Antibody
Protein sna Antibody
Protein snail homolog Antibody
Protein snail homolog 1 Antibody
SLUGH2 Antibody
SNA Antibody
Sna protein Antibody
SNAH Antibody
SNAI Antibody
SNAI1_HUMAN Antibody
SNAIL Antibody
Snail 1 homolog Antibody
Snail 1 zinc finger protein Antibody
Snail homolog 1 (Drosophila) Antibody
SNAIL, Drosophila, homolog of, 1 Antibody
SNAIL1 Antibody
Zinc finger protein SNAI1 Antibody
Information: Target information shown above is from the UniProt Consortium.
Western Blot - Anti-SNAI1 Antibody from ABclonal (A5544) - Antibodies.com
Western blot analysis of extracts of various cells, using SNAI1 antibody.
Please Note: All batches are extensively validated and are guaranteed to work as specified, however, QC data is not necessarily from the current lot on sale.

   Please Note: These publications cite this antibody under different brand names & catalogue numbers.


Cites ab180714

Mahmoud MM  et al. Shear stress induces endothelial-to-mesenchymal transition via the transcription factor Snail. Sci Rep 7:3375 (2017).

Cites ab180714

Deneka AY  et al. Tumor-targeted SN38 inhibits growth of early stage non-small cell lung cancer (NSCLC) in a KRas/p53 transgenic mouse model. PLoS One 12:e0176747 (2017).

Cites ab180714

Saito H  et al. Potential of extravasated platelet aggregation as a surrogate marker for overall survival in patients with advanced gastric cancer treated with preoperative docetaxel, cisplatin and S-1: a retrospective observational study. BMC Cancer 17:294 (2017).

Cites ab180714

Bian XL  et al. Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation. Nat Commun 8:14420 (2017).

Cites ab180714

Fang S  et al. Cisplatin promotes mesenchymal-like characteristics in osteosarcoma through Snail. Oncol Lett 12:5007-5014 (2016).

Cites ab180714

Zhou W  et al. Nrf2 inhibits epithelial-mesenchymal transition by suppressing snail expression during pulmonary fibrosis. Sci Rep 6:38646 (2016).

Cites ab180714

Mitchell B  et al. BRAF and epithelial-mesenchymal transition in papillary thyroid carcinoma - challenging the roles of Snail and E-Cadherin? Am J Transl Res 8:5076-5086 (2016).

Cites ab180714

Yang S  et al. STC2 promotes head and neck squamous cell carcinoma metastasis through modulating the PI3K/AKT/Snail signaling. Oncotarget 8:5976-5991 (2017).

Cites ab180714

Tanaka N  et al. Acquired platinum resistance involves epithelial to mesenchymal transition through ubiquitin ligase FBXO32 dysregulation. JCI Insight 1:e83654 (2016).

Cites ab180714

Jaca A  et al. The role of miRNA-21 and epithelial mesenchymal transition (EMT) process in colorectal cancer. J Clin Pathol 70:331-356 (2017).

Cites ab180714

Feng D  et al. Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis. Oncotarget 7:64575-64588 (2016).

Cites ab180714

Li C  et al. Overexpression of angiopoietin 2 promotes the formation of oral squamous cell carcinoma by increasing epithelial-mesenchymal transition-induced angiogenesis. Cancer Gene Ther 23:295-302 (2016).

Cites ab180714

Wei MG  et al. JiaWeiDangGui Decoction Ameliorates Proteinuria and Kidney Injury in Adriamycin-Induced Rat by Blockade of TGF-ß/Smad Signaling. Evid Based Complement Alternat Med 2016:5031890 (2016).

Cites ab180714

Blanco S  et al. Stem cell function and stress response are controlled by protein synthesis. Nature 534:335-40 (2016).

Cites ab180714

Kudinov AE  et al. Musashi-2 (MSI2) supports TGF-ß signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis. Proc Natl Acad Sci U S A 113:6955-60 (2016).

Cites ab180714

Kugler J  et al. Editor's Highlight: Identification and Characterization of Teratogenic Chemicals Using Embryonic Stem Cells Isolated From a Wnt/ß-Catenin-Reporter Transgenic Mouse Line. Toxicol Sci 152:382-94 (2016).

Cites ab180714

Ding H  et al. Glioma-associated oncogene homolog 1 promotes epithelial-mesenchymal transition in human renal tubular epithelial cell. Am J Transl Res 8:662-9 (2016).

Cites ab180714

Ma X  et al. Baicalein suppresses metastasis of breast cancer cells by inhibiting EMT via downregulation of SATB1 and Wnt/ß-catenin pathway. Drug Des Devel Ther 10:1419-41 (2016).

Cites ab180714

Bai X  et al. MicroRNA-130b improves renal tubulointerstitial fibrosis via repression of Snail-induced epithelial-mesenchymal transition in diabetic nephropathy. Sci Rep 6:20475 (2016).

Cites ab180714

Xiang Z  et al. CXCL1 expression is correlated with Snail expression and affects the prognosis of patients with gastric cancer. Oncol Lett 10:2458-2464 (2015).

Cites ab180714

Jiang FZ  et al. Mutant p53 induces EZH2 expression and promotes epithelial-mesenchymal transition by disrupting p68-Drosha complex assembly and attenuating miR-26a processing. Oncotarget 6:44660-74 (2015).

Cites ab180714

Tan H  et al. miR-382 inhibits migration and invasion by targeting ROR1 through regulating EMT in ovarian cancer. Int J Oncol 48:181-90 (2016).

Cites ab180714

Zheng X  et al. Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Nature 527:525-30 (2015).

Cites ab180714

Qin W  et al. Inhibition of autophagy promotes metastasis and glycolysis by inducing ROS in gastric cancer cells. Oncotarget 6:39839-54 (2015).

Cites ab180714

Xie B  et al. DDR2 facilitates hepatocellular carcinoma invasion and metastasis via activating ERK signaling and stabilizing SNAIL1. J Exp Clin Cancer Res 34:101 (2015).

Cites ab180714

Cicchini C  et al. Epigenetic control of EMT/MET dynamics: HNF4a impacts DNMT3s through miRs-29. Biochim Biophys Acta 1849:919-29 (2015).

Cites ab180714

Xu X  et al. Snail Is a Direct Target of Hypoxia-inducible Factor 1a (HIF1a) in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells. J Biol Chem 290:16653-64 (2015).

Cites ab180714

Piskareva O  et al. The development of cisplatin resistance in neuroblastoma is accompanied by epithelial to mesenchymal transition in vitro. Cancer Lett N/A:N/A (2015).

Cites ab180714

Wei MG  et al. Ferulic Acid Attenuates TGF-ß1-Induced Renal Cellular Fibrosis in NRK-52E Cells by Inhibiting Smad/ILK/Snail Pathway. Evid Based Complement Alternat Med 2015:619720 (2015).

Cites ab180714

Li Z  et al. The long non-coding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer. J Transl Med 13:84 (2015).

Cites ab180714

Yin X  et al. Coexpression of gene Oct4 and Nanog initiates stem cell characteristics in hepatocellular carcinoma and promotes epithelial-mesenchymal transition through activation of Stat3/Snail signaling. J Hematol Oncol 8:23 (2015).

Rating

Species

Application

Date

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Mouse

IHC-P

12-Oct-2015

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Human

ICC/IF

07-Aug-2015

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Human

ICC/IF

29-Sep-2014

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Human

WB

26-Sep-2014

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Mouse

WB

21-Aug-2014

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Mouse

IHC-Fr

03-Jun-2014


Total Citations (1)
Description: Goat polyclonal antibody to SNAI1.
Application: ELISA, WB, IHC
Host: Goat
Isotype: IgG
Reactivity: Human, Rat

£205

Description: Rabbit polyclonal antibody to SNAI1.
Application: WB, IHC
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat

£148 – £372

Description: Rabbit polyclonal antibody to SNAI1 (Phospho-Ser246)
Application: WB, IF
Host: Rabbit
Reactivity: Human, Mouse
Immunogen: Peptide sequence around phosphorylation site of Serine 246(T-F-S(p)-R-M) derived from Human SNAI1.

£180 – £234

Description: Rabbit polyclonal antibody to SNAI1
Application: WB, IHC
Host: Rabbit
Reactivity: Human
Immunogen: Full length fusion protein

£234

Please Note: Anti-SNAI1 Antibody is for research use only. It is not intended for diagnostic or therapeutic use.
Antibodies.com | A5544
Size Price Quantity
50µl £148
100µl £243
200µl £372

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