Anti-SOX2 Antibody (A12651)

Name: Anti-SOX2 Antibody
Brand: Antibodies.com
SKU: A12651
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SOX2 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Tags:

iPSCs Marker Neural Stem Cell Marker Neuroepithelial Marker Radial Glia Marker

Specifications

Name

Anti-SOX2 Antibody

Description

Rabbit polyclonal antibody to SOX2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SOX2 (NP_003097.1).

Sequence

MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ANOP3, cb236, Delta EF2a, lcc, MCOPS3, MGC148683, MGC2413, RGD1565646, Sex determining region Y box 2, SOX 2, SOX2_HUMAN, SRY (sex determining region Y) box 2, SRY box containing gene 2, SRY related HMG box 2, SRY related HMG box gene 2, SRY-box 2, Transcription factor SOX 2, Transcription factor SOX-2, ysb

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-SOX2 Antibody (A12651)

Western blot analysis of extracts of MCF7 cells, using Anti-SOX2 Antibody (A12651) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-SOX2 Antibody (A12651)

Western blot analysis of extracts of various cell lines, using Anti-SOX2 Antibody (A12651) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-SOX2 Antibody (A12651)

Immunohistochemistry analysis of paraffin-embedded mouse embryos using Anti-SOX2 Antibody (A12651) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-SOX2 Antibody (A12651)

Immunofluorescence analysis of F9 cells using Anti-SOX2 Antibody (A12651) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).