Anti-SPAM1 Antibody [ARC56311] (A306582)

Name: Anti-SPAM1 Antibody [ARC56311]
Brand: Antibodies.com
SKU: A306582

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This product is discontinued

Anti-SPAM1 Antibody [ARC56311] (A306582) has been discontinued and is no longer available.

View all Anti-SPAM1 Antibodies.

Specifications

Name

Anti-SPAM1 Antibody [ARC56311]

Description

Rabbit monoclonal [ARC56311] antibody to SPAM1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 61-223 of human SPAM1 (NP_694859.1).

Sequence

LGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC56311

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

58 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

epididymis secretory sperm binding protein Li 96n, HEL-S-96n, HYA1, HYAL PH-20, HYAL PH20, Hyal-PH20, HYAL1, HYAL3, HYAL5, HYALP_HUMAN, Hyaluronidase PH-20, Hyaluronidase PH20, Hyaluronoglucosaminidase PH-20, MGC26532, PH-20 Hyaluronidase, PH20, PH20 Hyaluronidase, SPAG15, SPAM-1, Sperm adhesion molecule 1, sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding), Sperm surface protein PH-20, Sperm surface protein PH20, zona pellucida binding

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-SPAM1 Antibody [ARC56311] (A306582)

Western blot analysis of Rat testis, using Anti-SPAM1 Antibody [ARC56311] (A306582) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-SPAM1 Antibody [ARC56311] (A306582)

Western blot analysis of Mouse testis, using Anti-SPAM1 Antibody [ARC56311] (A306582) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-SPAM1 Antibody [ARC56311] (A306582)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-SPAM1 Antibody [ARC56311] (A306582) at a dilution of 1:800(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SPAM1 Antibody [ARC56311] (A306582)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-SPAM1 Antibody [ARC56311] (A306582) at a dilution of 1:800(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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