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Anti-STAT1 Antibody [SM1] (A85870)

Flow Cytometry - Anti-STAT1 Antibody [SM1] (A85869) - Antibodies.com
This product is discontinued

Anti-STAT1 Antibody [SM1] (A85870) has been discontinued and is no longer available.

View all STAT1 Antibodies.

  • Specifications

Name
Anti-STAT1 Antibody [SM1]
Description
Mouse monoclonal [SM1] antibody to STAT1.
Specificity
The antibody recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Applications
IP, WB, IHC-P
Dilutions
IHC-P: 5 µg/ml; Positive Control: lung macrophages, WB: Recommended dilution 1-2 µg/ml.
Reactivity
Mouse, Human
Immunogen
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
Sequence
DNLLPMSPEEFDE
Host
Mouse
Clonality
Monoclonal
Clone ID
SM1
Isotype
IgG2b
Conjugate

Unconjugated

Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
91 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84
Isotype Controls
Suitable Secondaries
See all Anti-Mouse IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Flow Cytometry - Anti-STAT1 Antibody [SM1] (A85869) - Antibodies.comEnlarge Image

Flow Cytometry - Anti-STAT1 Antibody [SM1] (A85870)

Induction of phosphorylation of STAT1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT1 (Ser727) by the Anti-STAT1 Antibody [PSM1] (upper panel) and total STAT1 level by the Anti-STAT1 Antibody [SM1] (lower panel).

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Proteins predicted to interact with STAT1

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score