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Anti-Superoxide Dismutase 1 Antibody (A305066)

Western Blot - Anti-Superoxide Dismutase 1 Antibody (A305066) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A305066) - Antibodies.com
$465
Product Datasheet

Rabbit polyclonal antibody to Superoxide Dismutase 1 for WB, ICC/IF and ELISA.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Superoxide Dismutase 1 Antibody
Description
Rabbit polyclonal antibody to Superoxide Dismutase 1.
Specificity
This antibody recognises a conformation specific epitope where the beta barrel is unfolded.
Applications
WB, ICC/IF, ELISA
Dilutions
WB: 1:1,000, ICC/IF: 1:100
Reactivity
Human, Mouse, Rat
Immunogen
Within 50 AA of N-terminal region of human SOD1, unfolded beta barrel region.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Protein A purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ALS, ALS1, Amyotrophic Lateral Sclerosis 1 (Adult), Amyotrophic lateral sclerosis 1 adult, Cu,Zn Superoxide Dismutase, EC 1.15.1.111, Cu/Zn SOD, Cu/Zn superoxide dismutase, Epididymis secretory protein Li 44, HEL S 44, Homodimer, hSod1, Indophenoloxidase A, IPOA, Mn superoxide dismutase, SOD, SOD soluble, SOD1, SOD2, SODC, SODC_HUMAN, Superoxide dismutase 1 soluble, Superoxide Dismutase 1, Soluble, Superoxide dismutase Cu Zn, Superoxide dismutase cystolic, Superoxide dismutase [Cu-Zn], Superoxide Dismutase, Cystolic
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Superoxide Dismutase 1 Antibody (A305066) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Superoxide Dismutase 1 Antibody (A305066)
Western blot analysis of Human, rat, mouse Hela cells, Brain, lung showing detection of ~18 kDa SOD1 (UBB) protein using Anti-Superoxide Dismutase 1 Antibody (A305066) at 1:1,000. Lane 1: Molecular Weight Ladder. Lane 2: human HeLa Cell Lysates. Lane 3: rat Brain. Lane 4: mouse Lung. Load: 20 µg. Predicted/Observed Size: ~18 kDa.
Immunocytochemistry/Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A305066) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Superoxide Dismutase 1 Antibody (A305066)
Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Superoxide Dismutase 1 Antibody (A305066), at 1:110 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Cytoplasm. Nucleus. Mitochondrion. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-SOD1 (UBB) Antibody. (C) Composite.
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