Anti-Tau (phospho Ser416) Antibody (A308895)

Name: Anti-Tau (phospho Ser416) Antibody
Brand: Antibodies.com
SKU: A308895
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Tau (phospho Ser416) for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Tags:

Cytoplasm Marker Cytoskeleton Marker Mitochondrial Marker Plasma Membrane Marker

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Specifications

Name

Anti-Tau (phospho Ser416) Antibody

Description

Rabbit polyclonal antibody to Tau (phospho Ser416).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S416 of human Tau (NP_005901.2).

Sequence

TGSID

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

95 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI413597, AW045860, DDPAC, FLJ31424, FTDP 17, G protein beta1/gamma2 subunit interacting factor 1, MAPT, MAPTL, MGC134287, MGC138549, MGC156663, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, Microtubule-associated protein tau, MSTD, Mtapt, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament tau, Paired helical filament-tau, PHF tau, PHF-tau, PPND, PPP1R103, Protein phosphatase 1, regulatory subunit 103, pTau, RNPTAU, Tauopathy and respiratory failure, included, TAU_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Tau (phospho Ser416) Antibody (A308895)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-Tau (phospho Ser416) Antibody (A308895) at 1:1,000 dilution or Tau antibody (A0002). NIH/3T3 cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Tau (phospho Ser416) Antibody (A308895)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-Tau (phospho Ser416) Antibody (A308895) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Tau (phospho Ser416) Antibody (A308895)

Immunofluorescence analysis of rat brain using Anti-Tau (phospho Ser416) Antibody (A308895) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Tau (phospho Ser416) Antibody (A308895)

Immunofluorescence analysis of mouse brain using Anti-Tau (phospho Ser416) Antibody (A308895) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

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