Mouse monoclonal [JOVI.1] antibody to TCR beta Constant 1 (APC).
Specificity
This antibody recognizes an extracellular epitope on TCR C beta 1.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR V beta 3 C beta 1.
Host
Mouse
Clonality
Monoclonal
Clone ID
JOVI.1
Isotype
IgG2a
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human TCR Cbeta1 positive lymphocytes (red-filled) from TCR Cbeta1 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using Anti-TCR C beta 1 Antibody (APC) (4 µl reagent / 100 µl of peripheral whole blood).
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