Anti-Tissue Factor Antibody [ARC60559] (A309523)

Name: Anti-Tissue Factor Antibody [ARC60559]
Brand: Antibodies.com
SKU: A309523

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This product is discontinued

Anti-Tissue Factor Antibody [ARC60559] (A309523) has been discontinued and is no longer available.

View all Anti-Tissue Factor Antibodies.

Specifications

Name

Anti-Tissue Factor Antibody [ARC60559]

Description

Rabbit monoclonal [ARC60559] antibody to Tissue Factor.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human Coagulation factor III/Tissue Factor.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC60559

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

45 - 53 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD142, CD142 antigen, Coagulation factor III, Coagulation factor III (thromboplastin tissue factor), F3, FLJ17960, TF, TFA, TF_HUMAN, Thromboplastin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Tissue Factor Antibody [ARC60559] (A309523)

Western blot analysis of various lysates, using Anti-Tissue Factor Antibody [ARC60559] (A309523) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Tissue Factor Antibody [ARC60559] (A309523)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Tissue Factor Antibody [ARC60559] (A309523) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Tissue Factor Antibody [ARC60559] (A309523)

Immunofluorescence analysis of A-431 and MCF7(Negative sample) cells using Anti-Tissue Factor Antibody [ARC60559] (A309523) at a dilution of 1:300 (40x lens). DAPI was used to stain the cell nuclei (blue).

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