Recombinant Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free (A324269)

Name: Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free
Brand: Antibodies.com
SKU: A324269
Price: 295.0 USD
Availability: InStock

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$295

Recombinant human monoclonal [CDP571] antibody to TNF alpha for ELISA, FACS, Functional Assay and In Vivo.

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Freight Charges

$40 to United States

Lead Time

7-10 Business Days

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+1 (314) 370-6046

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Tags:

Recombinant Antibody Endosomal Marker Nuclear Marker Plasma Membrane Marker

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Specifications

Name

Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free

Description

Recombinant human monoclonal [CDP571] antibody to TNF alpha.

Applications

ELISA, FACS, Functional Assay, In Vivo

Reactivity

Human

Host

Human

Clonality

Monoclonal

Clone ID

CDP571

Isotype

IgG4SP - Human IgG4 with S228P mutation

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A affinity chromatography.

Concentration

Reconstitution dependent.

Molecular Weight

This antibody has a predicted MW of 146.9 kDa.

Purity

> 90% (by SDS-PAGE and SEC-HPLC).

Product Form

Lyophilized

Reconstitution

Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!

Formulation

Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Synonyms

APC1, APC1 protein, Cachectin, DIF, Differentiation inducing factor, Macrophage cytotoxic factor, soluble form, Tnf, TNF a, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2 , TNF superfamily, member 2, TNF, macrophage derived, TNF, monocyte derived, TNF-a, TNF-alpha, TNFA, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor (TNF superfamily member 2) , Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor, Membrane Form, Tumor necrosis factor, soluble form

Suitable Secondaries

See all Anti-Human IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: SDS-PAGE - Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free (A324269)

SDS-PAGE analysis of Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free (A324269) under reducing (R) conditions confirms that the purity of this antibody is greater than 90%.

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Figure 2: Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free (A324269)

SEC-HPLC analysis of Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free (A324269) confirms that the purity of this antibody is greater than 95%.

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Figure 3: Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free (A324269)

Immobilized recombinant human TNF alpha protein (Fc tag) is bound by Anti-TNF alpha Antibody [CDP571] - Low endotoxin, Azide free (A324269). EC50 was approximately 0.05253 µg/ml.

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