Anti-TOMM20 Antibody (A88476)

Name: Anti-TOMM20 Antibody
Brand: Antibodies.com
SKU: A88476
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to TOMM20 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody Mitochondrial Marker

Specifications

Name

Anti-TOMM20 Antibody

Description

Rabbit polyclonal antibody to TOMM20.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOM20 (NP_055580.1).

Sequence

YCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

KIAA0016, MAS20, MGC117367, Mitochondrial 20 kDa outer membrane protein, Mitochondrial import receptor subunit TOM20 homolog, MOM19, Outer mitochondrial membrane receptor Tom20, TOM20, TOM20_HUMAN, Translocase of outer mitochondrial membrane 20 homolog (yeast), Translocase of outer mitochondrial membrane 20 homolog type II

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-TOMM20 Antibody (A88476)

Western blot analysis of extracts of various cell lines, using Anti-TOMM20 Antibody (A88476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5min.

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Figure 2: Western Blot - Anti-TOMM20 Antibody (A88476)

Western blot analysis of extracts from normal (control) and TOM20 knockout (KO) 293T cells, using Anti-TOMM20 Antibody (A88476) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-TOMM20 Antibody (A88476)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-TOMM20 Antibody (A88476) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-TOMM20 Antibody (A88476)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-TOMM20 Antibody (A88476) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.