Western Blot - Anti-p53 (acetyl Lys382) Antibody (A91514)
Western blot analysis of extracts of various cell lines, using Anti-p53 (acetyl Lys382) Antibody (A91514) at 1:1,000 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 180s.
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