Anti-TRPV4 Antibody [ARC58721] (A309503)

Name: Anti-TRPV4 Antibody [ARC58721]
Brand: Antibodies.com
SKU: A309503

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This product is discontinued

Anti-TRPV4 Antibody [ARC58721] (A309503) has been discontinued and is no longer available.

View all Anti-TRPV4 Antibodies.

Specifications

Name

Anti-TRPV4 Antibody [ARC58721]

Description

Rabbit monoclonal [ARC58721] antibody to TRPV4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:2,000-1:6,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human TRPV4.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC58721

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

BCYM3, CMT2C, HMSN2C, osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OSM9 like transient receptor potential channel 4, Osmosensitive transient receptor potential channel 4, OTRPC 4, OTRPC4, SMAL, SPSMA, SSQTL1, Transient receptor potential cation channel subfamily V member 4, Transient receptor potential protein 12, TRP 12, TRP12, TRPV 4, TRPV4_HUMAN, Vanilloid receptor like channel 2, Vanilloid receptor like protein 2, Vanilloid receptor related osmotically activated channel, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR 4, VR OAC, VR-OAC, VR4, VRL 2, VRL-2, VRL2, VROAC

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-TRPV4 Antibody [ARC58721] (A309503)

Western blot analysis of A375, using Anti-TRPV4 Antibody [ARC58721] (A309503) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:5,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-TRPV4 Antibody [ARC58721] (A309503)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-TRPV4 Antibody [ARC58721] (A309503) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-TRPV4 Antibody [ARC58721] (A309503)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-TRPV4 Antibody [ARC58721] (A309503) at a dilution of 1:400 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-TRPV4 Antibody [ARC58721] (A309503)

Immunofluorescence analysis of mouse kidney using Anti-TRPV4 Antibody [ARC58721] (A309503) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).