Anti-Ube2B Antibody [ARC2512] (A305883)

Name: Anti-Ube2B Antibody [ARC2512]
Brand: Antibodies.com
SKU: A305883
Price: 470.0 USD
Availability: InStock

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This product is discontinued

Anti-Ube2B Antibody [ARC2512] (A305883) has been discontinued and is no longer available.

View all Anti-Ube2B Antibodies.

Specifications

Name

Anti-Ube2B Antibody [ARC2512]

Description

Rabbit monoclonal [ARC2512] antibody to Ube2B.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 53-152 of human UBE2B (P63146).

Sequence

FKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC2512

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

17 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E2 17kDa, E2 protein, hHR6B, HR6B, RAD6 homolog B, RAD6B, UBC2, UBE2B_HUMAN, Ubiquitin carrier protein B, Ubiquitin conjugating enzyme E2 B, Ubiquitin conjugating enzyme E2B, Ubiquitin conjugating enzyme E2B (RAD6 homolog), Ubiquitin protein ligase B, Ubiquitin-conjugating enzyme E2 17 kDa, Ubiquitin-conjugating enzyme E2 B, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase B

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Ube2B Antibody [ARC2512] (A305883)

Western blot analysis of extracts of Rat testis, using Anti-Ube2B Antibody [ARC2512] (A305883) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Ube2B Antibody [ARC2512] (A305883)

Western blot analysis of extracts of various cell lines, using Anti-Ube2B Antibody [ARC2512] (A305883) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunohistochemistry - Anti-Ube2B Antibody [ARC2512] (A305883)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Ube2B Antibody [ARC2512] (A305883) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Ube2B Antibody [ARC2512] (A305883)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Ube2B Antibody [ARC2512] (A305883) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.