Anti-Ubiquitin Antibody [5B9-B3] (A305078)

Name: Anti-Ubiquitin Antibody [5B9-B3]
Brand: Antibodies.com
SKU: A305078
Price: 510.0 USD
Availability: InStock

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$510

Mouse monoclonal [5B9-B3] antibody to Ubiquitin for WB, ICC/IF and ELISA.

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$40 to United States

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5-8 Business Days

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Lysosome Marker

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Anti-Ubiquitin Antibody [Ubi-1]

Mouse monoclonal (Ubi-1) antibody to Ubiquitin for WB, ICC/IF and IHC.

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Anti-Ubiquitin Antibody

Rabbit polyclonal antibody to Ubiquitin for WB, ICC/IF and IHC.

Specifications

Name

Anti-Ubiquitin Antibody [5B9-B3]

Description

Mouse monoclonal [5B9-B3] antibody to Ubiquitin.

Applications

WB, ICC/IF, ELISA

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine

Immunogen

Native bovine ubiquitin, conjugated to KLH.

Host

Mouse

Clonality

Monoclonal

Clone ID

5B9-B3

Isotype

IgG2a

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~10 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epididymis secretory protein Li 50, FLJ25987, HEL S 50, MGC8385, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBB_HUMAN, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin B, UBIQ_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Ubiquitin Antibody [5B9-B3] (A305078)

Western blot analysis of human cell lysates showing detection of Ubiquitin protein using Anti-Ubiquitin Antibody [5B9-B3] (A305078) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

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Figure 2: Immunofluorescence - Anti-Ubiquitin Antibody [5B9-B3] (A305078)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Ubiquitin Antibody [5B9-B3] (A305078), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.

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Figure 3: Immunofluorescence - Anti-Ubiquitin Antibody [5B9-B3] (A305078)

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Ubiquitin Antibody [5B9-B3] (A305078), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.

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