Anti-Ubiquitin Antibody [ARC50024] (A307666)

Name: Anti-Ubiquitin Antibody [ARC50024]
Brand: Antibodies.com
SKU: A307666
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC50024] antibody to Ubiquitin for WB, IHC and ICC/IF.

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$40 to United States

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5-8 Business Days

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Lysosome Marker

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Anti-Ubiquitin Antibody

Rabbit polyclonal antibody to Ubiquitin for WB, ICC/IF and IHC.

Specifications

Name

Anti-Ubiquitin Antibody [ARC50024]

Description

Rabbit monoclonal [ARC50024] antibody to Ubiquitin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human Ubiquitin (AAH46123.1).

Sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC50024

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 - 300 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Epididymis secretory protein Li 50, FLJ25987, HEL S 50, MGC8385, Polyubiquitin B, RPS 27A, RPS27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBB_HUMAN, UBC, UBCEP 1, UBCEP 2, UBCEP1, UBCEP2, Ubiquitin B, UBIQ_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Ubiquitin Antibody [ARC50024] (A307666)

Western blot analysis of extracts of various cell lines, using Anti-Ubiquitin Antibody [ARC50024] (A307666) at 1:1,000 dilution. HeLa, NIH/3T3 and C6 cell were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 2: Immunohistochemistry - Anti-Ubiquitin Antibody [ARC50024] (A307666)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Ubiquitin Antibody [ARC50024] (A307666) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Ubiquitin Antibody [ARC50024] (A307666)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Ubiquitin Antibody [ARC50024] (A307666) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Ubiquitin Antibody [ARC50024] (A307666)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Ubiquitin Antibody [ARC50024] (A307666) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.