Anti-V5 Tag Antibody (A121614)

Name: Anti-V5 Tag Antibody
Brand: Antibodies.com
SKU: A121614
Price: 535.0 USD
Availability: InStock

1 Citations

Enlarge Image

$535

Goat polyclonal antibody to V5 Tag for WB.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-V5 Tag Antibody

Description

Goat polyclonal antibody to V5 Tag.

Specificity

The antibody recognizes the V5 tag fused to either the amino- or carboxy-terminal of recombinant proteins.

Applications

Dilutions

WB: 1:500-1:5,000, IF: 1:500-1:2,000

Immunogen

Recombinant peptide corresponding to amino acids 95-108 of the P and V proteins of the Paramyxovirus of simian virus SV5, expressed in and purified from E. coli.

Sequence

GKPIPNPLLGLDST

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

3 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 20% Glycerol and 0.05% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, rev, V5 epitope tag

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

Enlarge Image

Figure 1: Western Blot - Anti-V5 Tag Antibody (A121614)

MBP-V5 recombinant protein and 293 cells, transfected with V5-mCherry, detected with Anti-V5 Tag Antibody at a 1:1,000 dilution. Lysates at 100µg per lane and rabbit anti-goat IgG antibody (HRP) at a 1:10,000 dilution.

Enlarge Image

Figure 2: Anti-V5 Tag Antibody (A121614)

Myo5a deficient melanocytes transiently transduced with adenovirus expressing V5-tagged Myo5a, fixed, and stained with Anti-V5 Tag Antibody at a 1:1,000 dilution. Donkey anti-goat IgG antibody (Alexa Fluor 568) was used as the secondary antibody.

Citations (1)

View Publication

The Na⁺ -coupled glucose transporter SGLT2 interacts with its accessory unit MAP17 in vitro and their expressions overlap in the renal proximal tubule.

References: Anti-V5 Tag Antibody (A121614)

Abstract:

Na⁺ -glucose cotransporter 2 is the renal Na⁺ -coupled glucose transporter responsible for the tubular glucose reabsorption, while MAP17 was recently identified as its accessory unit. Mutations in either of the proteins' coding genes, SLC5A2 and PDZK1IP1, lead to urinary glucose excretion. To investigate whether MAP17 interacts with SGLT2 in vitro, we engineered a V5-tagged SGLT2 construct and evaluated HEK293T cells coexpressing it together with a HA tagged MAP17 construct. MAP17 is shown to colocalize and coimmunoprecipitate with SGLT2. Also, in human kidney sections, the expression of both proteins overlaps at the apical surface of tubular epithelia. This interaction provides the rationale behind SGLT2 activation by MAP17 as well the similarity of the SLC5A2 and PDZK1IP1 glucosuric phenotypes.

View Publication

Publishing research using Anti-V5 Tag Antibody (A121614)? Please let us know so that we can list the citation on this page.