Anti-V5 Tag Antibody [SV5-Pk2] (A281787)

$295

Mouse monoclonal [SV5-Pk2] antibody to V5 Tag for WB, IP and ELISA.

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-V5 Tag Antibody [SV5-Pk2]
Description
Mouse monoclonal [SV5-Pk2] antibody to V5 Tag.
Specificity
This antibody recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk2 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
Applications
WB, IP, ELISA
Dilutions
WB: 1:100 - 1:500
Reactivity
Viral
Immunogen
A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
Host
Mouse
Clonality
Monoclonal
Clone ID
SV5-Pk2
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti V5-Tag antibody, clone SV5-Pk2 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk2 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
Synonyms
GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, rev, V5 epitope tag
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-V5 Tag Antibody [SV5-Pk2] (A281787)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-V5 Tag Antibody [SV5-Pk2] (A281787)

Top