Anti-Versican Antibody [S351-23] (A305042)

Name: Anti-Versican Antibody [S351-23]
Brand: Antibodies.com
SKU: A305042
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S351-23] antibody to Versican for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Versican Antibody [S351-23]

Description

Mouse monoclonal [S351-23] antibody to Versican.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 362-585 (glycosaminoglycan alpha domain) of mouse Versican core protein.

Host

Mouse

Clonality

Monoclonal

Clone ID

S351-23

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

>350 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Chondroitin sulfate proteoglycan 2, Chondroitin sulfate proteoglycan core protein 2, Chondroitin sulfate proteoglycan core protein, cartilage, CSPG2, CSPG2_HUMAN, ERVR, GHAP, Glial hyaluronate binding protein, Glial hyaluronate-binding protein, Large fibroblast proteoglycan, PG-M, PGM, VCAN, Versican core protein, Versican proteoglycan, WGN, WGN 1, WGN1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Versican Antibody [S351-23] (A305042)

Western blot analysis of rat brain membrane and brain showing detection of 350kDa Versican protein using Anti-Versican Antibody [S351-23] (A305042) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain Membrane. Lane 3: rat Brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: KPL TMB. Predicted/Observed Size: 350kDa.

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Figure 2: Immunofluorescence - Anti-Versican Antibody [S351-23] (A305042)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-Versican Antibody [S351-23] (A305042), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Versican Antibody (D) Composite.

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Figure 3: Immunofluorescence - Anti-Versican Antibody [S351-23] (A305042)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Versican Antibody [S351-23] (A305042), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm, Membrane, Extracellular Space, Extracellular Matrix. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Versican Antibody. (D) Composite.

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Figure 4: Western Blot - Anti-Versican Antibody [S351-23] (A305042)

Western blot analysis of rat brain membrane and brain showing detection of 350kDa Versican protein using Anti-Versican Antibody [S351-23] (A305042) at 1:200 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder. Lane 2: rat brain membrane and brain. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:1,000 for 1 hour room temperature. Color Development: KPL TMB. Predicted/Observed Size: 350kDa. Other Band(s): Multiple other bands.

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