Anti-Vinculin Antibody (A88162)

Name: Anti-Vinculin Antibody
Brand: Antibodies.com
SKU: A88162
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Vinculin for WB and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Vinculin Antibody

Description

Rabbit polyclonal antibody to Vinculin.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 767-1066 of human Vinculin (NP_003364.1).

Sequence

AKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

124 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC, VINC_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Vinculin Antibody (A88162)

Western blot analysis of extracts of various cell lines, using Anti-Vinculin Antibody (A88162) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-Vinculin Antibody (A88162)

Immunofluorescence analysis of PC-12 cells using Anti-Vinculin Antibody (A88162) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-Vinculin Antibody (A88162)

Immunofluorescence analysis of U2OS cells using Anti-Vinculin Antibody (A88162) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Vinculin Antibody (A88162)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Vinculin Antibody (A88162) at a dilution of 1:150. DAPI was used to stain the cell nuclei (blue).

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