Anti-Vinculin Antibody [ARC51900] (A305534)

Name: Anti-Vinculin Antibody [ARC51900]
Brand: Antibodies.com
SKU: A305534
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51900] antibody to Vinculin for WB, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Vinculin Antibody [ARC51900]

Description

Rabbit monoclonal [ARC51900] antibody to Vinculin.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:10,000-1:30,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-900 of human Vinculin (NP_054706.1).

Sequence

DAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMM

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51900

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

124 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC, VINC_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)

Western blot analysis of various lysates, using Anti-Vinculin Antibody [ARC51900] (A305534) at 1:250,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunofluorescence - Anti-Vinculin Antibody [ARC51900] (A305534)

Confocal imaging of HeLa cells using Anti-Vinculin Antibody [ARC51900] (A305534), at a dilution of 1:100, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.

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Figure 3: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Vinculin Antibody [ARC51900] (A305534). This Western blot was performed on the immunoprecipitate using Anti-Vinculin Antibody [ARC51900] (A305534) at a dilution of 1:1000.

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