Anti-AKT1 Antibody [ARC51585] (A308667)

Name: Anti-AKT1 Antibody [ARC51585]
Brand: Antibodies.com
SKU: A308667
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51585] antibody to AKT1 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-AKT1 Antibody [ARC51585]

Description

Rabbit monoclonal [ARC51585] antibody to AKT1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:1,000-1:5,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 381-480 of human AKT1 (P31749).

Sequence

SGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51585

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-AKT1 Antibody [ARC51585] (A308667)

Western blot analysis of various lysates, using Anti-AKT1 Antibody [ARC51585] (A308667) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 25s.

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Figure 2: Immunohistochemistry - Anti-AKT1 Antibody [ARC51585] (A308667)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-AKT1 Antibody [ARC51585] (A308667)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-AKT1 Antibody [ARC51585] (A308667)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-AKT1 Antibody [ARC51585] (A308667) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.