Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Name: Anti-AKT1 (phospho Ser473) Antibody [ARC0169]
Brand: Antibodies.com
SKU: A80471
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC0169] antibody to AKT1 (phospho Ser473) for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-AKT1 (phospho Ser473) Antibody [ARC0169]

Description

Rabbit monoclonal [ARC0169] antibody to AKT1 (phospho Ser473).

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S473 of human Akt1 (P31749).

Sequence

QFSYS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0169

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Western blot analysis of extracts of various cell lines, using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at 1:1,000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.