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Signal Transduction
Protein Phosphorylation
Serine & Threonine Kinases
PKB & AKT
Anti-Akt1 (Phospho-Tyr474) Antibody (A50308)
Overview
Specifications
Images
Enlarge Image
Enlarge Image
Enlarge Image
$265
Rabbit polyclonal antibody to Akt1 (Phospho-Tyr474) for WB, IHC and ELISA.
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100µl
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Specifications
Name
Anti-Akt1 (Phospho-Tyr474) Antibody
Description
Rabbit polyclonal antibody to Akt1 (Phospho-Tyr474)
Specificity
Phospho-Akt1 (Y474) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at Y474.
Applications
WB
,
IHC
,
ELISA
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Synthesized peptide derived from human Akt1 around the phosphorylation site of Y474.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1mg / ml
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20˚C / 1 year
Synonyms
AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Suitable Secondaries
Goat Anti-Rabbit IgG H&L Antibody (AP) (A294874)
Goat Anti-Rabbit IgG H&L Antibody (Biotin) (A294795)
Goat Anti-Rabbit IgG H&L Antibody (FITC) (A294887)
Goat Anti-Rabbit IgG H&L Antibody (HRP) (A294888)
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (3)
Enlarge Image
Figure 1: Anti-Akt1 (Phospho-Tyr474) Antibody (A50308)
Western Blot analysis of 293T cells using Phospho-Akt1 (Y474) Polyclonal Antibody
Enlarge Image
Figure 2: Anti-Akt1 (Phospho-Tyr474) Antibody (A50308)
Western Blot analysis of 293T 22RV1 cells using Phospho-Akt1 (Y474) Polyclonal Antibody
Enlarge Image
Figure 3: Anti-Akt1 (Phospho-Tyr474) Antibody (A50308)
Western Blot analysis of KB using Phospho-Akt1 (Y474) Polyclonal Antibody
Publishing research using Anti-Akt1 (Phospho-Tyr474) Antibody (A50308)? Please
let us know
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