Anti-Bcl-2 Antibody (A12540)

Name: Anti-Bcl-2 Antibody
Brand: Antibodies.com
SKU: A12540
Price: 335.0 USD
Availability: InStock

This product is identical to Abcam (ab194583). Discover more for less.

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$335

Rabbit polyclonal antibody to Bcl-2 for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Highlighted products for Anti-Bcl-2 Antibody

A86278

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Anti-Bcl-2 Antibody [Bcl-2/100]

Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.

A86278

16 Citations

Anti-Bcl-2 Antibody [Bcl-2/100]

Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.

Specifications

Name

Anti-Bcl-2 Antibody

Description

Rabbit polyclonal antibody to Bcl-2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2).

Sequence

MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

26 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Bcl-2 Antibody (A12540)

Western blot analysis of 293T, using Anti-Bcl-2 Antibody (A12540) at 1:900 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Bcl-2 Antibody (A12540)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Bcl-2 Antibody (A12540) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Bcl-2 Antibody (A12540)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Bcl-2 Antibody (A12540) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-Bcl-2 Antibody (A12540)

Immunoprecipitation analysis of 200µg extracts of THP-1 cells using 3µg of Anti-Bcl-2 Antibody (A12540). This Western blot was performed on the immunoprecipitate using Anti-Bcl-2 Antibody (A12540) at a dilution of 1:1000.

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