Anti-Bcl-2 Antibody [ARC0173] (A306910)

Name: Anti-Bcl-2 Antibody [ARC0173]
Brand: Antibodies.com
SKU: A306910
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0173] antibody to Bcl-2 for WB, IHC and IP.

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$40 to United States

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5-8 Business Days

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Anti-Bcl-2 Antibody [Bcl-2/100]

Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.

Specifications

Name

Anti-Bcl-2 Antibody [ARC0173]

Description

Rabbit monoclonal [ARC0173] antibody to Bcl-2.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human Bcl-2 (P10415).

Sequence

MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0173

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

26 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Bcl-2 Antibody [ARC0173] (A306910)

Western blot analysis of extracts of various cell lines, using Anti-Bcl-2 Antibody [ARC0173] (A306910) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)

Immunohistochemistry analysis of paraffin-embedded human tonsil (100X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue (100X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue (400X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.