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Anti-Bcl-2 (phospho Ser70) Antibody (A11053)

Western Blot - Anti-Bcl-2 (phospho Ser70) Antibody (A11053) - Antibodies.com Immunofluorescence - Anti-Bcl-2 (phospho Ser70) Antibody (A11053) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to Bcl-2 (phospho Ser70) for WB and ICC/IF.

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A86278
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Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
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A86278
16 Citations
Anti-Bcl-2 Antibody [Bcl-2/100]
Mouse monoclonal [Bcl-2/100] antibody to Bcl-2 for Flow Cytometry, IP, WB, IHC-P, IHC-Fr and ICC.
Name
Anti-Bcl-2 (phospho Ser70) Antibody
Description
Rabbit polyclonal antibody to Bcl-2 (phospho Ser70).
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Rat
Immunogen
A synthetic phosphorylated peptide around S70 of human Bcl-2 (NP_000624.2).
Sequence
RTSPL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Bcl-2 (phospho Ser70) Antibody (A11053) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Bcl-2 (phospho Ser70) Antibody (A11053)
Western blot analysis of extracts of Jurkat and 293 cells, using Anti-Bcl-2 (phospho Ser70) Antibody (A11053) at 1:1,000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
Immunofluorescence - Anti-Bcl-2 (phospho Ser70) Antibody (A11053) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Bcl-2 (phospho Ser70) Antibody (A11053)
Immunofluorescence analysis of HeLa cells using Anti-Bcl-2 (phospho Ser70) Antibody (A11053) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Bcl-2 (phospho Ser70) Antibody (A11053)? Please let us know so that we can list the citation on this page.

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