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Anti-BDNF Antibody [AMC0122] (A308198)

Western Blot - Anti-BDNF Antibody [AMC0122] (A308198) - Antibodies.com
$470
Product Datasheet

Mouse monoclonal [AMC0122] antibody to BDNF for WB.

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Rabbit polyclonal antibody to BDNF for WB and IHC.
Name
Anti-BDNF Antibody [AMC0122]
Description
Mouse monoclonal [AMC0122] antibody to BDNF.
Applications
WB
Dilutions
WB: 1:2,000-1:10,000
Reactivity
Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (P23560).
Sequence
PMKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHVIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR
Host
Mouse
Clonality
Monoclonal
Clone ID
AMC0122
Isotype
IgG2a
Light Chains
kappa
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Abrineurin, ANON2, BDNF_HUMAN, Brain Derived Neurotrophic Factor, Brain-derived neurotrophic factor, BULN2, MGC34632, Neurotrophin
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-BDNF Antibody [AMC0122] (A308198) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-BDNF Antibody [AMC0122] (A308198)
Western blot analysis of extracts of various cell lines, using Anti-BDNF Antibody [AMC0122] (A308198) at 1:10,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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