Anti-BDNF Antibody [ARC0303] (A309102)

Name: Anti-BDNF Antibody [ARC0303]
Brand: Antibodies.com
SKU: A309102
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0303] antibody to BDNF for WB and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Anti-BDNF Antibody

Rabbit polyclonal antibody to BDNF for WB and IHC.

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Anti-BDNF Antibody

Rabbit polyclonal antibody to BDNF for WB and IHC.

Specifications

Name

Anti-BDNF Antibody [ARC0303]

Description

Rabbit monoclonal [ARC0303] antibody to BDNF.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human BDNF (P23560).

Sequence

VPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGID

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0303

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

15 kDa / 28 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Abrineurin, ANON2, BDNF_HUMAN, Brain Derived Neurotrophic Factor, Brain-derived neurotrophic factor, BULN2, MGC34632, Neurotrophin

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-BDNF Antibody [ARC0303] (A309102)

Western blot analysis of extracts of THP-1 cells, using Anti-BDNF Antibody [ARC0303] (A309102) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-BDNF Antibody [ARC0303] (A309102)

Western blot analysis of extracts of various cell lines, using Anti-BDNF Antibody [ARC0303] (A309102) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 3: Immunofluorescence - Anti-BDNF Antibody [ARC0303] (A309102)

Immunofluorescence analysis of mouse brain using Anti-BDNF Antibody [ARC0303] (A309102) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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