Anti-beta Tubulin Antibody (A17307)

Name: Anti-beta Tubulin Antibody
Brand: Antibodies.com
SKU: A17307
Price: 230.0 USD
Availability: InStock

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$230

Rabbit polyclonal antibody to beta Tubulin for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-beta Tubulin Antibody

Description

Rabbit polyclonal antibody to beta Tubulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human ß-Tubulin (NP_006077.2).

Sequence

SDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, M40, MGC117247, MGC16435, OK/SW cl.56, OK/SWcl.56, TBB5_HUMAN, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-beta Tubulin Antibody (A17307)

Western blot analysis of various lysates, using Anti-beta Tubulin Antibody (A17307) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-beta Tubulin Antibody (A17307)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-beta Tubulin Antibody (A17307)Immunohistochemistry analysis of paraffin-embedded human liver using beta-Tubulin Rabbit polyclonal antibody (AC008) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-beta Tubulin Antibody (A17307)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-beta Tubulin Antibody (A17307)Immunohistochemistry analysis of paraffin-embedded human lung cancer using beta-Tubulin Rabbit polyclonal antibody (AC008) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-beta Tubulin Antibody (A17307)

Immunofluorescence analysis of C6 cells using Anti-beta Tubulin Antibody (A17307) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).