Anti-beta Tubulin Antibody [AMC0498] (A80389)

Name: Anti-beta Tubulin Antibody [AMC0498]
Brand: Antibodies.com
SKU: A80389
Price: 265.0 USD
Availability: InStock

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$265

Mouse monoclonal [AMC0498] antibody to beta Tubulin for WB, IHC, ICC/IF and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-beta Tubulin Antibody [AMC0498]

Description

Mouse monoclonal [AMC0498] antibody to beta Tubulin.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:5,000-1:10,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50- 1:200

Reactivity

Human, Mouse, Rat, Hamster, Monkey, Goat, Chlamydomonas Reinhardtii

Immunogen

Recombinant protein of human ß-Tubulin.

Host

Mouse

Clonality

Monoclonal

Clone ID

AMC0498

Isotype

IgG1

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

50 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 1% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Beta 4 tubulin, Beta 5 tubulin, beta Ib tubulin, Beta1 tubulin, Class I beta tubulin, M40, MGC117247, MGC16435, OK/SW cl.56, OK/SWcl.56, TBB5_HUMAN, TUBB, TUBB 1, TUBB 2, TUBB 5, TUBB1, TUBB2, TUBB5, tubulin beta 1 chain, Tubulin beta 2 chain, tubulin beta 5 chain, Tubulin beta chain, Tubulin beta class I, tubulin beta polypeptide, Tubulin beta-5 chain

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Western blot analysis of extracts of various cell lines, using Anti-beta Tubulin Antibody [AMC0498] (A80389) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-beta Tubulin Antibody [AMC0498] (A80389)Immunohistochemistry analysis of paraffin-embedded rat ovary using beta-Tubulin Mouse mAb (AC021) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Immunohistochemistry analysis of paraffin-embedded rat stomach using Anti-beta Tubulin Antibody [AMC0498] (A80389)Immunohistochemistry analysis of paraffin-embedded rat stomach using beta-Tubulin Mouse mAb (AC021) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-beta Tubulin Antibody [AMC0498] (A80389)

Immunofluorescence analysis of C6 cells using Anti-beta Tubulin Antibody [AMC0498] (A80389) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).