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Anti-c-Myc (phospho Ser62) Antibody (A12455)

Western Blot - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.com Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.com Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.com Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to c-Myc (phospho Ser62) for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-c-Myc (phospho Ser62) Antibody
Description
Rabbit polyclonal antibody to c-Myc (phospho Ser62).
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A phospho specific peptide corresponding to residues surrounding S62 of human MYC.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
62 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AU016757, Avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL, Myc, Myc Epitope Tag, Myc protein, Myc proto oncogene protein, Myc proto-oncogene protein, myc related translation/localization regulatory factor, myc-related translation/localization regulatory factor, Myc2, MYCC, MYC_HUMAN, Myelocytomatosis oncogene, Niard, Nird, oncogene c-Myc, Oncogene Myc, OTTHUMP00000158589, Proto-oncogene c-Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, v myc avian myelocytomatosis viral oncogene homolog, v myc myelocytomatosis viral oncogene homolog, V-Myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-c-Myc (phospho Ser62) Antibody (A12455)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-c-Myc (phospho Ser62) Antibody (A12455) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455)
Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-c-Myc (phospho Ser62) Antibody (A12455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-c-Myc (phospho Ser62) Antibody (A12455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-c-Myc (phospho Ser62) Antibody (A12455)
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-c-Myc (phospho Ser62) Antibody (A12455) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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