Anti-c-Myc Antibody [9E10] (A278887)

Anti-c-Myc Antibody [9E10]

Mouse monoclonal [9E10] antibody to c-Myc.

This antibody recognises the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence.

WB, IHC-P, IHC-Fr, ELISA, Flow Cytometry

ELISA: 1:100 - 1:500, FC 1: Neat for 20 µg, 0.1 mg, 1 mg, 1:10 for 2 mg - 1:10 for 20 µg, 0.1 mg, 1 mg, 1:50 for 2 mg, WB 2: 1:100 - 1:500, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human, Epitope tag

Synthetic peptide corresponding to amino acids 408-439 from the C-terminus of human c-myc conjugated to Keyhole Limpet Haemocyanin.

VVILKKATAYILSVQAEEQKLISEEDLLRKRR

Mouse

Monoclonal

9E10

IgG1

Unconjugated

Protein G affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti c-myc antibody, clone 9E10 detects the p62c-myc proto-oncogene protein, which is involved in the regulation of the cell cycle and cell growth. C-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines (Craig et al. 1993). Overexpression of c-myc has been reported in a wide variety of human cancers (Nesbit et al. 1999).Mouse anti c-myc antibody, clone 9E10 recognizes the sequence EQKLISEEDL and may be used to detect proteins and peptides labelled with molecular tags containing this sequence (Hilpert et al. 2001).

AU016757, Avian myelocytomatosis viral oncogene homolog, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39, MRTL, Myc, Myc Epitope Tag, Myc protein, Myc proto oncogene protein, Myc proto-oncogene protein, myc related translation/localization regulatory factor, myc-related translation/localization regulatory factor, Myc2, MYCC, MYC_HUMAN, Myelocytomatosis oncogene, Niard, Nird, oncogene c-Myc, Oncogene Myc, OTTHUMP00000158589, Proto-oncogene c-Myc, Protooncogene homologous to myelocytomatosis virus, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, v myc avian myelocytomatosis viral oncogene homolog, v myc myelocytomatosis viral oncogene homolog, V-Myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)