Rabbit polyclonal antibody to Calnexin (phospho Ser583).
Specificity
This antibody detects endogenous levels of Calnexin only when phosphorylated at Ser583.
Applications
WB, IHC, IF, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Calnexin around the phosphorylation site of Ser583 (amino acids 543-592).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
67kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CALX_HUMAN, CANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Figure 1: Western Blot - Anti-Calnexin (phospho Ser583) Antibody (A94794)
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30' using Anti-Calnexin (phospho Ser583) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-Calnexin (phospho Ser583) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-Calnexin (phospho Ser583) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.