Anti-Calreticulin Antibody [6C6] (A85411)

HeLa cell cultures were stained with Anti-Calreticulin Antibody (red). Calreticulin predominately localized in vesicles and the ER. Cells were counterstained with Anti-Vimentin Antibody (A85421 | green). Blue is a DNA stain.

Immunofluorescent analysis of of SH-SY5Y cells stained with Anti-Calreticulin Antibody [6C6] (A85411), at a dilution of 1:500, in green, and co-stained with Anti-Lamin A + C Antibody (A85443), at a dilution of 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The Anti-Calreticulin Antibody [6C6] (A85411) reveals granular staining of cytoplasm, while the Anti-Lamin A + C Antibody (A85443) stains the nuclear lamina and membrane.

Western blot analysis of lysates of different cell lines using Anti-Calreticulin Antibody [6C6] (A85411), at a dilution of 1:2,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] NIH-3T3 cells, [3] HEK293 cells, [4] HeLa cells, [5] SH-SY5Y cells, [6] C6 cells, and [7] COS-1 cells. A strong single band at about 50 kDa corresponds to the calreticulin protein.

Western blot analysis of Anti-Calreticulin Antibody in HEK293 (Lane 1), 3T3 (Lane 2), SHSY-5Y (Lane 3) and HeLa cells (Lane 4). The Anti-Calreticulin Antibody binds strongly to calreticulin in 50 kDa.