Anti-Calreticulin Antibody (A305091)

Name: Anti-Calreticulin Antibody
Brand: Antibodies.com
SKU: A305091
Price: 425.0 USD
Availability: InStock

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$425

Rabbit polyclonal antibody to Calreticulin for WB, IHC, ICC/IF, IP and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Cytoplasm Marker Cytoskeleton Marker ER Marker Nuclear Marker

Specifications

Name

Anti-Calreticulin Antibody

Description

Rabbit polyclonal antibody to Calreticulin.

Applications

WB, IHC, ICC/IF, IP, Flow Cytometry

Dilutions

WB: 1:1,000, IHC: 1:100, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Monkey, Porcine, Hamster, Rabbit, Sheep

Immunogen

Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~63 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Autoantigen RO, CALR, CALR protein, Calregulin, CALR_HUMAN, cC1qR, CRP55, CRT, CRTC, Endoplasmic reticulum resident protein 60, Epididymis secretory sperm binding protein Li 99n, ERp60, FLJ26680, grp60, HACBP, HEL S 99n, RO, Sicca syndrome antigen A, Sicca syndrome antigen A (autoantigen Ro; calreticulin), SSA

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Immunofluorescence - Anti-Calreticulin Antibody (A305091)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Calreticulin Antibody (A305091), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 2: Immunohistochemistry - Anti-Calreticulin Antibody (A305091)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution. The Primary Antibody used was Anti-Calreticulin Antibody (A305091) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at room temperature. Localization: Cytoplasmic granule. Endoplasmic reticulum lumen.

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Figure 3: Western Blot - Anti-Calreticulin Antibody (A305091)

Western blot analysis of multiple cell lines lysates showing detection of Calreticulin protein using Anti-Calreticulin Antibody (A305091) at 1:5,000 for 2 hours at room temperature. Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Donkey Anti-Rabbit IgG: HRP for 1 hour at room temperature.

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Figure 4: Immunofluorescence - Anti-Calreticulin Antibody (A305091)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Calreticulin Antibody (A305091), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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