Human Growth Hormone Anti-Dug Assay ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Growth Hormone in serum and plasma.
Assay Type
Direct Sandwich (Quantitative)
Principle of Assay
Human Growth Hormone Anti-Drug Antibody ELISA Kit is an indirect ELISA that provides quantitative determination of antibodies targeting Growth Hormone in serum and plasma. Immobilized growth hormone captures anti-hGH antibodies, which are detected using enzyme-linked reagents and colorimetric measurement.
Components
This kit contains: A coated 96 well microtiter plate, 1.8 mL Calibrator diluent, 12 µL Calibrator (1.15 mg/mL), 10X 50 mL wash buffer, 50 mL Assay buffer, 1000X 17 µL detection reagent, 12 mL TMB, 12 mL TMB stop solution.
Platform
Pre-coated Microplate (12 x 8 well strips)
Detection Type
Colorimetric (HRP, OD450)
Sample Type
Serum and plasma.
Sensitivity
30 ng/mL
Product Range
30 ng/mL -1920 ng/mL
Assay Time
2.5 hours
Sample Size
15 ul
Reactivity
Human
Precision
Intra-assay CV: < 20%, Inter-assay CV: < 20%
General Notes
Samples should be collected as serum or plasma using EDTA or heparin anticoagulants and stored frozen at or below -20 °C for up to one year. Prior to assay, calibrators and test samples are diluted 1:100 with assay buffer by adding 5 µL of sample to 495 µL of assay buffer. Mix well and use immediately. Do not store diluted samples.
Storage
Shipped at +4°C. Store the ELISA standard at -20°C. Store all other reagents at +4°C