Human Growth Hormone ELISA Kit (A1441)

Human Growth Hormone ELISA Kit

Human Growth Hormone ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Growth Hormone in cell culture supernatant, serum and plasma.

Sandwich (quantitative)

Human Growth Hormone ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Growth Hormone in the samples. A monoclonal antibody specific for human Growth Hormone has been immobilized onto microwells. Standard or samples are pipetted into the wells, and Growth Hormone present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for Growth Hormone is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Growth Hormone bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Microplate

Colorimetric

0.62 pg/ml

62.50 - 4000 pg/ml

This kit can assay both native and recombinant human Growth Hormone.

Human

This ELISA kit has shown no significant cross-reactivity.

105% (Average Recovery from Serum Samples).

Store at +4°C. Do not use past expiration date!

GH, GH-N, GH1, Growth hormone 1, Pituitary growth hormone, Somatotropin

Information online is representative. Always refer to the Product Manual inside the kit.