Recombinant rabbit monoclonal [DM70] antibody to 2B4.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM70
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Synonyms
h2B4, NAIL, Natural killer cell receptor 2B4, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, NKR2B4, Signaling lymphocytic activation molecule 4, SLAM family member 4, SLAMF4
Anti-2B4 Antibody [DM70] - BSA and Azide free (A317394)
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human 2B4 Protein (Fc Chimera 6xHis Tag) (A318398) can bind Anti-2B4 Antibody [DM70] - Azide free (A317394) in a linear range of 1-100 ng/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human 2B4 (B) were surface stained with Anti-2B4 Antibody [DM70] - Azide free (A317394) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-2B4 Antibody [DM70] - Azide free (A317394) on THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different rabbit monoclonal Anti-2B4 Antibodies by titration of different concentrations onto THP-1 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Publishing research using Recombinant Anti-2B4 Antibody [DM70] - BSA and Azide free (A317394)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-2B4 Antibody [DM70] - BSA and Azide free (A317394)
![ELISA - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_1.png?profile=product_top)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_2.png?profile=product_top)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_4.png?profile=product_top)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_5.png?profile=product_top)
![ELISA - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_1.png?profile=product_top_thumb)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_2.png?profile=product_top_thumb)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_4.png?profile=product_top_thumb)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_5.png?profile=product_top_thumb)
![ELISA - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_1.png?profile=product_image)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_2.png?profile=product_image)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_4.png?profile=product_image)
![Flow Cytometry - Anti-2B4 Antibody [DM70] - Azide free (A317394) - Antibodies.com](https://cdn.antibodies.com/image/catalog/317/A317394_5.png?profile=product_image)
![ELISA - Anti-2B4 Antibody [DM69] - BSA and Azide free (A318631) - Antibodies.com](https://cdn.antibodies.com/image/catalog/318/A318631_1.png?profile=product_alternative)
