The antibody detects endogenous level of total 4E-BP1 protein.
Applications
WB, IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around aa.43~47 (S-T-T-P-G) derived from Human 4E-BP1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
eIF4E-binding protein 1, EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Ab-45) Antibody #21216 (left) or the same antibody preincubated with blocking peptide (right).
