Synthetic peptide within amino acids 75 and the C-terminus of human eIF4EBP1 (NP_004086. 1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
4E-BP1, eIF4E-binding protein 1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Sample: FFPE section of human breast carcinoma. Antibody: Anti-eIF4EBP1 Antibody (A295580) was used at a dilution of 1:20,000 ( 0.05µg/ml). Detection: DAB.
Samples: Whole cell lysate (100 µg) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody: Anti-eIF4EBP1 Antibody (A295580) was used at 0.25 µg/ml. Detection: Chemiluminescence.
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