AGRP expression in Human Hypothalamus analyzed by immunohistochemistry. Tissue was cryosectioned after PFA perfusion fixation, and antigen retrieval was achieved by steaming in citrate buffer, pH 6, at 80°C for 30 minutes. Staining was performed with Anti-AGRP Antibody (A83375) at 0.05µg/ml and revealed with a biotinylated Anti-goat secondary antibody, avidin-conjugated horseradish peroxidase (HRP), and Ni-DAB.
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