Anti-AMPK alpha 1 + AMPK alpha 2 (phospho Trp183 & phospho Trp172) Antibody (A16447)

AMPK alpha 1 + AMPK alpha 2 expression in C2C12 cell lysates analyzed by western blot. Cells were treated with Hydrogen Peroxide (2 nM) at 37°C for 15 minutes following serum starvation overnight. Primary antibody incubation was performed for 1 hour on 25ug protein per lane with Anti-AMPK alpha 1 + AMPK alpha 2 (Phospho T183 & Phospho T172) Antibody (A16447) at a dilution of 1:1000 and detected with chemiluminescence.

AMPK alpha 1 + AMPK alpha 2 expression in 293T cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 30ug protein per lane with Anti-AMPK alpha 1 + AMPK alpha 2 (Phospho T183 & Phospho T172) Antibody (A16447) at a dilution of 1:1000 and detected with chemiluminescence.

AMPK alpha 1 + AMPK alpha 2 expression in C2C12 cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 30ug protein per lane with Anti-AMPK alpha 1 + AMPK alpha 2 (Phospho T183 & Phospho T172) Antibody (A16447) at a dilution of 1:1000 and detected with chemiluminescence.

AMPK alpha 1 + AMPK alpha 2 expression in C6 cell lysates analyzed by western blot. Primary antibody incubation was performed for 1 hour on 30ug protein per lane with Anti-AMPK alpha 1 + AMPK alpha 2 (Phospho T183 & Phospho T172) Antibody (A16447) at a dilution of 1:1000 and detected with chemiluminescence.